apc-cy7-conjugated anti-cd27  (Becton Dickinson)

Journal:

Article Title: Patterns of Cytokine Production in Human Immunodeficiency Virus Type 1 (HIV-1)-Specific Human CD8 + T Cells after Stimulation with HIV-1-Infected CD4 + T Cells

doi: 10.1128/JVI.79.19.12536-12543.2005

Figure Lengend Snippet: CD27 and CD28 expression in HIV-1-specific CTL clones

Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated anti-human CD27, phycoerythrin (PE)-conjugated anti-human IFN-γ, ECD-conjugated anti-human CD28, allophycocyanin (APC)-conjugated anti-human TNF-α, APC-conjugated anti-human IFN-γ, PE Cy7-conjugated anti-human TNF-α, and APC Cy7-conjugated anti-human CD27 MAbs were purchased from PharMingen (San Diego, CA).

Techniques: Expressing

Journal:

Article Title: Patterns of Cytokine Production in Human Immunodeficiency Virus Type 1 (HIV-1)-Specific Human CD8 + T Cells after Stimulation with HIV-1-Infected CD4 + T Cells

doi: 10.1128/JVI.79.19.12536-12543.2005

Figure Lengend Snippet: Coexpression of three cytokines in freshly isolated HIVGag28-36-specific CD8+ T cells responding to HIV-1-infected CD4+ T cells. Intracellular cytokine production of Gag28-36-specific CD8+ T cells was measured 6 h after stimulation with CD4+ T cells infected with the Nef+ HIV-1 clone NL-432gagHXB2. Intracellular staining for the three cytokines IFN-γ, MIP-1β, and TNF-α was simultaneously carried out using the three MAbs specific for these cytokines, and then the expression of these cytokines was analyzed by flow cytometry. The CD8+ tetramer-positive cells are gated to determine both the frequency of cells expressing these cytokines and the CD27 and CD28 expression. The frequency is shown as a percentage of the total number of cells in a 3-dimensional presentation. The frequency of cells expressing these cytokines among total cytokine-producing cells is also shown in a pie chart. Cells expressing these cytokines are shown as follows: none of the three cytokines (gray), IFN-γ only (red), MIP-1β only (green), TNF-α only (blue), IFN-γ and MIP-1β (yellow), IFN-γ and TNF-α (violet), MIP-1β and TNF-α (cyan), and all three cytokines (black).

Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated anti-human CD27, phycoerythrin (PE)-conjugated anti-human IFN-γ, ECD-conjugated anti-human CD28, allophycocyanin (APC)-conjugated anti-human TNF-α, APC-conjugated anti-human IFN-γ, PE Cy7-conjugated anti-human TNF-α, and APC Cy7-conjugated anti-human CD27 MAbs were purchased from PharMingen (San Diego, CA).

Techniques: Isolation, Infection, Staining, Expressing, Flow Cytometry